Journal: Micromachines

Article Title: Rapid Detection and Trapping of Extracellular Vesicles by Electrokinetic Concentration for Liquid Biopsy on Chip

doi: 10.3390/mi9060306

Figure Lengend Snippet: ( a ) Fabrication and assembly of the microfluidic concentrator chip. Step 1: print PEDOT:PSS on the circular pattern of PDMS device directly. Step 2: flip the chip and reversibly attach the device on the SuperEpoxy 3 glass slide. Step 3: apply vacuum suction to enhance adhesion and electrodes were placed at the sample reservoir and the buffer reservoir for electrical voltage to start ICP. ( b ) A photograph of the fully assembled concentrator showing a single microchannel (17 µm high; 1 cm long; 200 µm wide) in PDMS with a reservoir (4 mm of diameter) at each end and a SEM image of a row of pillars (diameter ~10 µm; height ~17 µm) to confine the printed PEDOT:PSS solution on the PDMS.

Article Snippet: As presented in a (step 1), we built a single concentrator device by printing a conductive polymer poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) using GIX microplotter II (Sonoplot Inc., Middleton, WI, USA) on a PDMS device that works as a cation-selective membrane.

Techniques: